KOMP 312 Project Gene Information

Cavin3

Name:	caveolae associated 3
Resources:	MGI:1923422 NCBI:109042 PubMed, Gene Cloud, Additional resources at KOMP
Products:	vector, ESC, sperm

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SHIRPA Observations in Viewing Jar

Body Position

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Active
	Inactive
	Excessive Activity

Coat Appearance

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Tidy - well groomed
	Irregularities (piloerect, etc)

Defecation

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Present
	Absent

Head Bobbing

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Absent
	Present

Head Flicking

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Absent
	Present

Lacrimation

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Absent
	Present

Palpebral Closure

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Eyes open
	Eyes closed

Tremor

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Absent
	Present

Whiskers

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Present
	Absent

FOB Arousal

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Normal (alert, exploratory bhvr)
	Very high (hyperalert, excited, sudden mvmts)
	Somewhat High (slight excitement, tense, darting)
	Low (some hd and body mvmt)
	Somewhat low (some exploration with immobility )
	Very low (coma, stupor)

SHIRPA Behavior Recorded in Arena

Gait

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Fluid ~ 3mm pelvic elevation
	Lack of fluidity

Startle Response (from FOB)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Freezes or whole body startle
	Slight reactions (evidence noise was heard)
	Moderate reaction
	No reaction
	Exaggerated (jumps, bites, attacks)

Tail Elevation

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Horizontal extension
	Dragging
	Elevated/straub tail

Touch Escape

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Flees prior to touch
	No response
	Response to touch

Transfer Arousal

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Brief freeze (2-5 sec) then movement
	Extended Freeze (over 5 sec)
	Immediate movement (< 1 sec freeze)

FOB Clonic Movements

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Normal
	Quivers of limbs, ears, head or skin
	Mild tremors
	wet dog shakes
	Clonic convulsions
	Repetitive mvmts of mouth and jaws
	Severe or whole body tremors
	Myoclonic jerks

FOB Gait

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Normal
	Ataxia (Excessive sway, rocks or lurches)
	Feet markedly point outward
	Walks on tiptoes
	Body drags or flattened on surface
	Hind limbs (drag, or splayed)
	Forelimbs drag, extended, not supporting
	Hunched or crouched body position

FOB Tonic Movements

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Normal
	Contractions of extensors (limbs rigid)
	Emprosthotonos (hd & body rigidly extended fwd
	Severe clonic &/or tonic convulsions
	Opisthotonos (hd & body rigidly arched back)
	Explosive jumps

SHIRPA Observations above Arena

Positional Passivity

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Tail - Struggles
	Supine - Struggles
	Neck - Struggles
	No struggle

Skin Color

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Pink
	Blanched
	Bright deep red flush

Tail Suspension (20 sec)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Normal splay of limbs
	Grabs ipsilateral leg
	Hindpaw clutch
	Other responses
	Turns up sides
	Grabs contralateral leg
	Forepaw clutch

Trunk Curl

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Absent
	Present

SHIRPA Reflexes

Corneal Reflex (tail restrain)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Present
	Absent

Pinna Reflex (do not restrain)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Present
	Absent

Other SHIRPA Tests

Biting

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	None
	Biting

Contact Righting Reflex

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Present
	Absent

Vocalization

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	None
	Vocalization

Geotaxis and Strength

Hanging Wire Grid Test (60 sec)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	> 1 min
	Falls immediately
	30s to 1 min
	0 to 30s
	Invalid

Vertical Screen Test (20 sec)

HET

HOM

WT Littermate

WT Pop

20%

40%

60%

80%

100%

	Climbs to the top of the screen in 20 sec
	Falls; or remains on the bottom of the screen upside down
	Climbs past middle in 20 sec
	Turns upward, does not climb to top

The KOMP Phenotyping Project is funded by an ARRA grant to UC Davis and CHORI.

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