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KOMP 2 Phenotyping Effort

FACS

Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.

Measurements: B Cells, CD4+ / CD8+, CD4+ T, CD4- CD8-, CD8+ T, Monocytes, NK Cells


Gender

Genotype


Exception Level: 95%


Legend

No Exceptions
Less exceptionsMore exceptions

Additional Resources


Assay Description

The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.

Currently displaying FACS quantile exceptions at 95%

C1qtnf1
C1qtnf3
Cd320
Cxcl17
Dll4
Il17b
Il17d
Il20
Sema4b
Txndc12

The KOMP Phenotyping Project is funded by an ARRA grant to UC Davis and CHORI.

Questions? Comments? Please contact us: 1-888-KOMP-MICE or service@komp.org